Figure 5.
Figure 5. Repo-Man and Sds22 are required for timely dephosphorylation of the microtubule attachment factor Dsn1. (A and B) Immunofluorescence staining with an antibody against Dsn1 phosphorylated at Ser100 (pS100; Welburn et al., 2010; A) or total Dsn1 (Kline et al., 2006; B). Representative example images of cells costained for pSer100 Dsn1 (A) or Dsn1 (B), kinetochores (CREST), and DNA (Hoechst 33342). Bars, 10 µm. (C and D) Quantification of phosphorylated Dsn1 or total Dsn1 on kinetochores. pSer100 Dsn1/CREST or Dsn1/CREST fluorescence intensity ratios were normalized to control siRNA–transfected prometaphase cells. Mean ± SEM. n = 3 experiments (>30 cells per experimental condition; 16 kinetochores per cell). **, P < 0.001, using a two-tailed Student’s t test.

Repo-Man and Sds22 are required for timely dephosphorylation of the microtubule attachment factor Dsn1. (A and B) Immunofluorescence staining with an antibody against Dsn1 phosphorylated at Ser100 (pS100; Welburn et al., 2010; A) or total Dsn1 (Kline et al., 2006; B). Representative example images of cells costained for pSer100 Dsn1 (A) or Dsn1 (B), kinetochores (CREST), and DNA (Hoechst 33342). Bars, 10 µm. (C and D) Quantification of phosphorylated Dsn1 or total Dsn1 on kinetochores. pSer100 Dsn1/CREST or Dsn1/CREST fluorescence intensity ratios were normalized to control siRNA–transfected prometaphase cells. Mean ± SEM. n = 3 experiments (>30 cells per experimental condition; 16 kinetochores per cell). **, P < 0.001, using a two-tailed Student’s t test.

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