Figure 1. RhoA and RhoC have different effects of cell morphology and directional migration. PC3 cells were transfected with indicated siRNAs. (A) Cell morphology after siRNA transfection or C3 transferase treatment. Arrows indicate RhoC-depleted cells with increased spread area. (B) F-actin in cells on Matrigel. Boxed regions are shown at higher magnification below. (C) Images from movies of GFP–β-actin–expressing cells. Arrows indicate lateral protrusions. (D) Protrusion dynamics. Boxed regions on top are shown from movie frames (1 frame/min) in the bottom panels (boxed regions). (E) Migration speed. (F) Forward migration index (FMI) in a chemotaxis assay. *, P < 0.001. (G) Cell images during chemotaxis. White arrows indicate broader lamellipodia in RhoC-depleted cells. Black arrows: show gradient direction. Bars: (A) 40 µm; (B, C, D, and G) 20 µm.
Figure 1.

RhoA and RhoC have different effects of cell morphology and directional migration. PC3 cells were transfected with indicated siRNAs. (A) Cell morphology after siRNA transfection or C3 transferase treatment. Arrows indicate RhoC-depleted cells with increased spread area. (B) F-actin in cells on Matrigel. Boxed regions are shown at higher magnification below. (C) Images from movies of GFP–β-actin–expressing cells. Arrows indicate lateral protrusions. (D) Protrusion dynamics. Boxed regions on top are shown from movie frames (1 frame/min) in the bottom panels (boxed regions). (E) Migration speed. (F) Forward migration index (FMI) in a chemotaxis assay. *, P < 0.001. (G) Cell images during chemotaxis. White arrows indicate broader lamellipodia in RhoC-depleted cells. Black arrows: show gradient direction. Bars: (A) 40 µm; (B, C, D, and G) 20 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal