Figure 8. Real-time PCR quantitation of IGF2 and H19 mRNA transcripts. IGF2, H19, and the housekeeping β-actin genes were coamplified from each cDNA synthesized from normal tissue (human breast and skin), control, and decoy CTCF–expressing cells in MCF7 (A), HBF1, and WSF7 cells (B). IGF2 and H19 were quantitated in duplicate for each sample and were determined by a ΔCT and Δ2ΔCT calculation with reference to human β-actin gene control. IGF2 and H19 expression was normalized and presented as the number by using the IGF2 and H19 level in controls (white) as 1 (tissue, n = 6; control, n = 6; treatment, n = 6). *, P < 0.05 relative to IGF2 and H19 expression in the MCF7 control (A) and fibroblast control (B). All data are presented as means ± SD of three independent experiments.
Figure 8.

Real-time PCR quantitation of IGF2 and H19 mRNA transcripts. IGF2, H19, and the housekeeping β-actin genes were coamplified from each cDNA synthesized from normal tissue (human breast and skin), control, and decoy CTCF–expressing cells in MCF7 (A), HBF1, and WSF7 cells (B). IGF2 and H19 were quantitated in duplicate for each sample and were determined by a ΔCT and Δ2ΔCT calculation with reference to human β-actin gene control. IGF2 and H19 expression was normalized and presented as the number by using the IGF2 and H19 level in controls (white) as 1 (tissue, n = 6; control, n = 6; treatment, n = 6). *, P < 0.05 relative to IGF2 and H19 expression in the MCF7 control (A) and fibroblast control (B). All data are presented as means ± SD of three independent experiments.

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