Figure 4. Intrachromosomal loop between IGF2 promoters and the ICR. (A) A schematic diagram of the EcoRI sites in the IGF2/H19 imprinting locus used for the 3C assay. Arrows under each EcoR1 site mark the orientation of the 3C-specific primers. (B and C) Loss of the long-range intrachromosomal loop between the IGF2 promoters and the ICR in decoy CTCF–expressing MCF7 cells (B) and human skin fibroblasts (C). ZF-Sss1, Sss1-CTCF–expressing cells; control, wild-type cells.
Figure 4.

Intrachromosomal loop between IGF2 promoters and the ICR. (A) A schematic diagram of the EcoRI sites in the IGF2/H19 imprinting locus used for the 3C assay. Arrows under each EcoR1 site mark the orientation of the 3C-specific primers. (B and C) Loss of the long-range intrachromosomal loop between the IGF2 promoters and the ICR in decoy CTCF–expressing MCF7 cells (B) and human skin fibroblasts (C). ZF-Sss1, Sss1-CTCF–expressing cells; control, wild-type cells.

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