In vitro binding of recombinant proteins with SUZ12. (A) A schematic diagram of GST fusion constructs. CTCF-FL, full-length CTCF; NT, N-terminal; ZF, zinc finger domain; CT, C-terminal. (B) Binding of CTCF domains with SUZ12. Input: reaction aliquots collected before the pull-down reaction and analyzed in parallel with the samples using Western blotting with an anti–human SUZ12 antibody. (C) A schematic diagram of His-tagged fusion constructs. CTCF-HIS, His-tagged full-length CTCF; ZF-Sss1-HIS, His-tagged ZF-Sss1; ZF-EGFP-HIS, His-tagged ZF-EGFP. (D) A lack of interaction between His-tagged CTCF decoy proteins and SUZ12. The purified decoy proteins were incubated with cell extracts, and SUZ12 interaction was detected by Western blotting.