Wnt canonical and Wnt-CaMKII noncanonical pathways are reciprocally inhibitory in AHACs. (A) Q-PCR for AXIN2 of primary AHACs treated with 100 ng/ml WNT-3A in the presence of the CaMKII inhibitor KN93 or its inactive analogue KN92 (10 µM). (B) DKK1 promoted intracellular calcium accumulation in P0 AHACs. The inhibition of the canonical pathway with DKK1 enhanced intracellular calcium accumulation induced by WNT-3A. In all the experiments, n = 4. Data in A were analyzed with ANOVA; data in B were analyzed with an unpaired t test. Error bars indicate mean ± SEM. *, P < 0.05; ***, P < 0.0005.