Lis1 is not stably associated with moving dynein cargos. (A) Kymographs of Lis1-GFP (top) and mCherry-Rab5/RabA endosomes (middle) in A. nidulans germlings. Bottom images are merged images of the top and middle images. (B) Bar graph of the percentage of endosomes colocalizing with dynein, Lis1, or dynactin. 93.4 ± 3.0% (SEM) of moving mCherry-Rab5/RabA endosomes colocalized with a dynein-3xGFP particle (n = 110 endosomes). 1.8 ± 1.0% of endosomes colocalized with a Lis1-GFP particle (n = 148 endosomes). 56.4 ± 5.9% of endosomes colocalized with a p25-GFP particle (n = 161 endosomes). (C) Kymographs of p25-GFP (top) and mCherry-Rab5/RabA endosomes (middle) in A. nidulans germlings. Frequent colocalization of p25-GFP and endosomes was observed. Bottom images are merged images of the top and middle images. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs. White dashed arrows highlight examples of colocalized events.