Figure 5. Lis1 is not required for dynein motility. (A) Kymographs of dynein-3xGFP particles reveal movements toward and away from the microtubule plus end in both wild-type and Lis1Δ hyphae. Yellow dashed arrows indicate discrete particle movements toward and away from the hyphal tip. The locations of microtubule plus ends in the hyphal tip are indicated by yellow plus signs. (B) Histograms showing anterograde velocities of dynein-3xGFP particles in wild-type and Lis1Δ hyphae. Mean velocities are 2.09 ± 0.70 µm/s (SD) in wild-type and 2.17 ± 0.52 µm/s in Lis1Δ hyphae (P = 0.197; n = 200 [wild type]; n = 144 [Lis1Δ]). (C) Histograms showing retrograde velocities of dynein-3xGFP particles in wild-type and Lis1Δ hyphae. Mean velocities are 2.38 ± 0.80 µm/s (SD) in wild-type and 2.30 ± 0.75 µm/s in Lis1Δ hyphae (P = 0.420, n = 200 [wild type]; n = 105 [Lis1Δ]).
Figure 5.

Lis1 is not required for dynein motility. (A) Kymographs of dynein-3xGFP particles reveal movements toward and away from the microtubule plus end in both wild-type and Lis1Δ hyphae. Yellow dashed arrows indicate discrete particle movements toward and away from the hyphal tip. The locations of microtubule plus ends in the hyphal tip are indicated by yellow plus signs. (B) Histograms showing anterograde velocities of dynein-3xGFP particles in wild-type and Lis1Δ hyphae. Mean velocities are 2.09 ± 0.70 µm/s (SD) in wild-type and 2.17 ± 0.52 µm/s in Lis1Δ hyphae (P = 0.197; n = 200 [wild type]; n = 144 [Lis1Δ]). (C) Histograms showing retrograde velocities of dynein-3xGFP particles in wild-type and Lis1Δ hyphae. Mean velocities are 2.38 ± 0.80 µm/s (SD) in wild-type and 2.30 ± 0.75 µm/s in Lis1Δ hyphae (P = 0.420, n = 200 [wild type]; n = 105 [Lis1Δ]).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal