![Figure 4. Lis1 is required for the distribution of peroxisomes and endosomes but is not essential for endosome motility in vivo. (A and B) In wild-type hyphae, GFP-Rab5/RabA–labeled endosomes (A) and mCherry-PTS1–labeled peroxisomes (B) are uniformly distributed (left images) and move bidirectionally, as shown in kymographs generated from time-lapse videos (right images). In Lis1Δ hyphae, endosomes (A) and peroxisomes (B) mislocalize to the hyphal tip (left images) and are largely immotile (right images). Dashed lines indicate the outline of the hyphae. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs. (C) Kymographs of GFP-labeled endosomes in Lis1Δ mutants show examples of Lis1-independent movements of endosomes. (D) Histograms of velocities of anterograde-directed endosomes from wild-type versus Lis1Δ strains. Mean velocities are 2.62 ± 0.83 µm/s (SD) in wild-type hyphae and 2.25 ± 0.85 µm/s in Lis1Δ hyphae (P = 0.0002; n = 250 [wild type]; n = 101 [Lis1Δ]). (E) Histogram of velocities of retrograde-directed endosomes from wild-type versus Lis1Δ strains. Mean velocities are 2.66 ± 0.77 µm/s (SD) in wild-type hyphae and 2.43 ± 0.81 µm/s in Lis1Δ hyphae (P = 0.0169; n = 250 [wild type]; n = 82 [Lis1Δ]).](https://cdn.rupress.org/rup/content_public/journal/jcb/197/7/10.1083_jcb.201112101/4/jcb_201112101_fig4.jpeg?Expires=1767669754&Signature=WUBxk3LW9Ybx2jrLT7~LF4ERYSlRaz8uQPCDq~q1KuFVsrmXt~zGrdR~tavI999VjlaL1pBTmoyAcroEy~6cddTBy1S3QIpwxpo9qm~zCkPK1DKk~PeyHJuIfdxwHFanLZb9SGBhUHNEvQUh2H1NTIpCRcz5OfWQUgR7W6pm7fJ6MkIeyvRrUGSkCDrvbKPEjRRVRTMoky0F5pxBHoOyNI60jz2DuAUSK6pOnw4as-LUZl2BJH7QE0atFMZEaFRtXk9cXiyXYkHkS4WQ7fuzmpr1fRR5VMEP7~ku~j2svcH2VCqrlig3v4248DoFwO8GBCoaKKRbzzpf2T388O7wLQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Lis1 is required for the distribution of peroxisomes and endosomes but is not essential for endosome motility in vivo. (A and B) In wild-type hyphae, GFP-Rab5/RabA–labeled endosomes (A) and mCherry-PTS1–labeled peroxisomes (B) are uniformly distributed (left images) and move bidirectionally, as shown in kymographs generated from time-lapse videos (right images). In Lis1Δ hyphae, endosomes (A) and peroxisomes (B) mislocalize to the hyphal tip (left images) and are largely immotile (right images). Dashed lines indicate the outline of the hyphae. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs. (C) Kymographs of GFP-labeled endosomes in Lis1Δ mutants show examples of Lis1-independent movements of endosomes. (D) Histograms of velocities of anterograde-directed endosomes from wild-type versus Lis1Δ strains. Mean velocities are 2.62 ± 0.83 µm/s (SD) in wild-type hyphae and 2.25 ± 0.85 µm/s in Lis1Δ hyphae (P = 0.0002; n = 250 [wild type]; n = 101 [Lis1Δ]). (E) Histogram of velocities of retrograde-directed endosomes from wild-type versus Lis1Δ strains. Mean velocities are 2.66 ± 0.77 µm/s (SD) in wild-type hyphae and 2.43 ± 0.81 µm/s in Lis1Δ hyphae (P = 0.0169; n = 250 [wild type]; n = 82 [Lis1Δ]).
