Endosomes and peroxisomes colocalize with moving dynein. (A and B) Kymographs were generated from time-lapse videos. Dynein-3xGFP (top) colocalizes with mCherry-Rab5/RabA–labeled endosomes (A) and mCherry-PTS1–labeled peroxisomes (B; middle) in wild-type hyphae. Bottom images show merged images with white dashed arrows highlighting examples of colocalized events. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs.