Figure 3. Loss of Fuz leads to disrupted anterograde IFT. (a) Still frame from a video of GFP-IFT20 in a Fuz KD cell (Video 5). The orange box indicates the region shown in the bottom image, depicting still frames from a time-lapse video revealing abnormally large and immotile IFT particles (red arrowheads) in a Fuz KD axoneme. The final frame is taken from the end of the time series (Video 6). The axoneme is outlined in yellow. (b) In a fixed control embryo, IFT particles (green) can be seen at low density in the axonemes of a multiciliated cell (red). (c) In a mildly affected Fuz KD embryo, IFT particles accumulate at high density throughout the axonemes. (d) GFP-IFT20 particles are static for >4 min in Fuz KD axonemes. Red brackets indicate especially clear examples. Bars, 4 µm.
Figure 3.

Loss of Fuz leads to disrupted anterograde IFT. (a) Still frame from a video of GFP-IFT20 in a Fuz KD cell (Video 5). The orange box indicates the region shown in the bottom image, depicting still frames from a time-lapse video revealing abnormally large and immotile IFT particles (red arrowheads) in a Fuz KD axoneme. The final frame is taken from the end of the time series (Video 6). The axoneme is outlined in yellow. (b) In a fixed control embryo, IFT particles (green) can be seen at low density in the axonemes of a multiciliated cell (red). (c) In a mildly affected Fuz KD embryo, IFT particles accumulate at high density throughout the axonemes. (d) GFP-IFT20 particles are static for >4 min in Fuz KD axonemes. Red brackets indicate especially clear examples. Bars, 4 µm.

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