The levels of ring canal–associated proteins are reduced in rngo^GE3956 germline clones. (a) Western blot of extracts from wild-type ovaries (left) and ovaries with rngo^GE3956 germline clones (glc, right). α-Tubulin was used as a loading control. (b and c) A control germline clone lacking GFP shows the same level of Hts-RC (blue) as the adjacent wild-type egg chamber (b), whereas Hts-RC levels are strongly reduced in an rngo^GE3956 germline clone egg chamber (c). (d–g) The same is true for PY epitopes (d and e, blue), Filamin (f and g, red), and Kel (f and g, blue). Germline clones are marked by the absence of GFP (green). Images are projections of z stacks. Egg chambers are at stages 5–7. Anterior is to the left. Bars, 20 µm.