Subcellular localization of Rngo during oogenesis. (a) Rngo (red) is expressed in somatic cells and germline cells of egg chambers. It localizes to the cytoplasm and to the nuclei. The strongest staining can be seen in the nucleus of the oocyte (arrows), marked by the expression of Orb (green). (b–d) Costaining of Rngo with lamin (b, green), ubiquitin (c, green), and with the proteasome (d, green). (e) Germline cells homozygously mutant for rngo^GE3956 (asterisk) completely lack Rngo staining. Clones are marked by loss of GFP expression (green). (f) L3 larval protein extracts were analyzed by Western blotting. In larvae possessing the wild-type rngo gene (right lanes), a band of 60 kD corresponding to FL Rngo is detectable, which is absent in the extract of rngo^GE3956 mutant animals (left lane). α-Tubulin was used as a loading control. In a–e, DNA was stained with DAPI (turquoise, only shown in the merged images). Egg chambers are at stages 6 and 7. Anterior is to the left. fc, follicle cell; nc, nurse cell; oc, oocyte. Bars: (a–d) 10 µm; (e) 20 µm.