Figure 6.
Figure 6. VASP phosphorylation regulates β1 integrin dynamics. (A) Example images of FRAP analysis of β1-GFP before and after bleaching. The arrow denotes the region of interest subjected to photobleaching. Bar, 10 µm. (B) Quantification of FRAP analysis of β1-GFP recovery in mock transfected (control) cells or those coexpressing WT, S153A, or S153D VASP-mCherry. τ1/2 values (s) were obtained from normalized recovery data for β1-GFP in each condition. Data are shown as mean values ± SEM from 12 cells from three independent experiments (indicated by error bars). **, P < 0.01 compared with recovery in control cells.

VASP phosphorylation regulates β1 integrin dynamics. (A) Example images of FRAP analysis of β1-GFP before and after bleaching. The arrow denotes the region of interest subjected to photobleaching. Bar, 10 µm. (B) Quantification of FRAP analysis of β1-GFP recovery in mock transfected (control) cells or those coexpressing WT, S153A, or S153D VASP-mCherry. τ1/2 values (s) were obtained from normalized recovery data for β1-GFP in each condition. Data are shown as mean values ± SEM from 12 cells from three independent experiments (indicated by error bars). **, P < 0.01 compared with recovery in control cells.

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