Figure 2.
Figure 2. The structural deformation of the inner kinetochore when cells are treated with MG132. (A) Immuno-EM images with anti–CENP-T antibodies in DT40 cells treated with nocodazole (Noc) or MG132. (B) Measurement of the length of the inner kinetochore containing CENP-T in cells treated with nocodazole (n = 100) or MG132 (n = 100). (C) Measurement of the width of the inner kinetochore containing CENP-T in cells treated with nocodazole (n = 100) or MG132 (n = 100). (D) Immuno-EM images with anti–CENP-C antibodies in DT40 cells treated with nocodazole or MG132. (E) Measurement of the length of the inner kinetochore containing CENP-C in cells treated with nocodazole (n = 100) or MG132 (n = 100). (F) Measurement of the width of the inner kinetochore containing CENP-C in cells treated with nocodazole (n = 100) or MG132 (n = 100). (G) Immuno-EM images with anti-HA antibodies in CENP-A–HA-expressing DT40 cells treated with nocodazole or MG132. (H) Measurement of the length of the inner kinetochore containing CENP-A–HA in cells treated with nocodazole (n = 70) or MG132 (n = 70). (I) Measurement of the width of the inner kinetochore containing CENP-A–HA in cells treated with nocodazole (n = 70) or MG132 (n = 70). (J) Immuno-EM images with anti–CENP-R antibodies in DT40 cells treated with nocodazole or MG132. (K) Measurement of the length of the inner kinetochore containing CENP-R in cells treated with nocodazole (n = 100) or MG132 (n = 100). (L) Measurement of the width of the inner kinetochore containing CENP-R in cells treated with nocodazole (n = 100) or MG132 (n = 100). Error bars show SDs, and the significance of these measurements was estimated by the Student’s t test (indicated by asterisks). Yellow boxes indicate the regions of higher magnification on the right. Bars, 250 nm.

The structural deformation of the inner kinetochore when cells are treated with MG132. (A) Immuno-EM images with anti–CENP-T antibodies in DT40 cells treated with nocodazole (Noc) or MG132. (B) Measurement of the length of the inner kinetochore containing CENP-T in cells treated with nocodazole (n = 100) or MG132 (n = 100). (C) Measurement of the width of the inner kinetochore containing CENP-T in cells treated with nocodazole (n = 100) or MG132 (n = 100). (D) Immuno-EM images with anti–CENP-C antibodies in DT40 cells treated with nocodazole or MG132. (E) Measurement of the length of the inner kinetochore containing CENP-C in cells treated with nocodazole (n = 100) or MG132 (n = 100). (F) Measurement of the width of the inner kinetochore containing CENP-C in cells treated with nocodazole (n = 100) or MG132 (n = 100). (G) Immuno-EM images with anti-HA antibodies in CENP-A–HA-expressing DT40 cells treated with nocodazole or MG132. (H) Measurement of the length of the inner kinetochore containing CENP-A–HA in cells treated with nocodazole (n = 70) or MG132 (n = 70). (I) Measurement of the width of the inner kinetochore containing CENP-A–HA in cells treated with nocodazole (n = 70) or MG132 (n = 70). (J) Immuno-EM images with anti–CENP-R antibodies in DT40 cells treated with nocodazole or MG132. (K) Measurement of the length of the inner kinetochore containing CENP-R in cells treated with nocodazole (n = 100) or MG132 (n = 100). (L) Measurement of the width of the inner kinetochore containing CENP-R in cells treated with nocodazole (n = 100) or MG132 (n = 100). Error bars show SDs, and the significance of these measurements was estimated by the Student’s t test (indicated by asterisks). Yellow boxes indicate the regions of higher magnification on the right. Bars, 250 nm.

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