Figure 2. Talin induces increased embedding of both ends of the β3 TMD. (A) Emission spectra of L694C-bimane nanodisc in the presence of increasing concentrations of THD. Fluorescence intensities were normalized to the maximum fluorescence intensity when no THD was present. (B) The emission spectra of I721C-bimane nanodiscs were analyzed as in A. (C) Nanodiscs containing truncated β3 TMD tail peptide were bimane labeled at I721C (ΔI721C-bimane), and response to THD was analyzed as in A. (A–C) All data are representative of at least three independent experiments. (D) Talin-induced topographic change of β3 TMD that can account for altered embedding. Orange cylinders indicate a β3 cytoplasmic tail, which forms a stable helix upon talin binding.
Figure 2.

Talin induces increased embedding of both ends of the β3 TMD. (A) Emission spectra of L694C-bimane nanodisc in the presence of increasing concentrations of THD. Fluorescence intensities were normalized to the maximum fluorescence intensity when no THD was present. (B) The emission spectra of I721C-bimane nanodiscs were analyzed as in A. (C) Nanodiscs containing truncated β3 TMD tail peptide were bimane labeled at I721C (ΔI721C-bimane), and response to THD was analyzed as in A. (A–C) All data are representative of at least three independent experiments. (D) Talin-induced topographic change of β3 TMD that can account for altered embedding. Orange cylinders indicate a β3 cytoplasmic tail, which forms a stable helix upon talin binding.

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