Quantitative analysis of aggregates of various Nck SH3 density. (A–E) Aggregates were divided into different groups based on their Nck SH3 density. Measured characteristics of individual aggregates were averaged and plotted against Nck SH3 density. Error bars indicate SEM of each density group. Significance is indicated by asterisks above data points (P < 0.05), with each notation indicating a significant difference between each set of data. The plots show that increasing Nck SH3 density results in a nonlinear increase in the velocity of aggregates (A), actin comet tail length (B), number of actin molecules in the comet tail core (C), the peak actin concentration in actin foci or comet tails (D), and the distance between the aggregate centroid and the peak actin concentration in aggregate-induced actin structures (E). These data are also presented as the experimental dataset in Fig. 5 to allow for comparison with model predictions.