Initiation sites were rarely detected in the subtelomere of Ch 7q. SMARD analysis of the Ch 7 telomere/subtelomere segment of human ES H9 cells is shown. Few initiation or termination events were detected in the molecules examined. The lack of distinct peaks or valleys in the replication profile histogram indicates the absence of preferred initiation or termination sites. Similar numbers of replication forks proceed in the telomere-to-subtelomere (3′-to-5′) and subtelomere-to-telomere (5′-to-3′) directions. Many of the 5′-to-3′ forks appear to initiate from origins at least 150 kb from the telomere. The yellow arrows indicate the direction of replication fork progression, and the vertical orange and blue lines demarcate the boundaries of sequences where fish probes bind, as described in the legend to Fig. 2.