Initiation events occur throughout the human 11q segment and forks move across the segment in both directions in three cell lines.SMARD analysis of the Ch11q telomere segment in human ES H9, H1, and IMR-90 fibroblast cells revealed similar program features. Frequent initiation events occurred throughout the full 140-kb length of the segment. Preferred sites of initiation, seen as peaks in the replication profile histograms, could be detected. In all three lines, the 11q telomere is replicated primarily by forks progressing from the subtelomere to the telomere. The yellow arrows indicate the direction of replication fork progression, and the vertical orange and blue lines demarcate the boundaries of sequences where fish probes bind, as described in the legend to Fig. 2.