KIR2DL2 photostimulation induces actin remodeling. (A–D) NKL cells expressing KIR2DL2 and Lifeact-RFP were imaged using TIRF microscopy and UV irradiated on bilayers containing ULBP3, ICAM, and either HLA-Cw3(cage) (A, C, and D) or HLA-Cw3(Tyr) (B). Photostimulation was performed using cells left untreated (A and B) or treated with NSC87877 (C) or Mn²⁺ (D). For each panel, a time-lapse montage (∼75-s intervals) is shown (top) along with an associated kymograph. UV irradiation is indicated by magenta text in the time lapse and by a magenta line in the kymograph. Kymographs were generated using the yellow line in the first image of each time lapse. Shown on the bottom in each panel, normalized mean fluorescence intensity of Lifeact-RFP in the center of the contact is graphed as a function of time together with cell area. The contact center is indicated by a cyan ellipse in each time lapse. Data are representative of at least two independent experiments. ΔF/F, normalized fluorescence intensity. Bars, 5 µm.