SRL1 mRNA colocalizes with Ssd1-9A. Cells expressing GAL-inducible Ssd1-9A-RFP and SRL1 mRNA-GFP (FLY3196 with plasmid FLE1244) were transferred to galactose medium for 1–2 h. SRL1 mRNA and Ssd1-9A localization were subsequently observed by spinning disk confocal microscopy. In all cells with detectable Ssd1-9A-RFP and SRL1 mRNA-GFP puncta, all SRL1 mRNA spots colocalized with Ssd1-9A puncta (100%, n = 45 cells). Parallel experiments established that nearly all Ssd1-9A puncta colocalize with P-bodies (see Fig. 7). The images represent single optical sections. Bar, 2 µm.