Asymmetric SRL1 mRNA localization is significantly diminished in ssd1Δ cells and rescued by phosphomimetic Ssd1-9D. SRL1 mRNA was GFP tagged and analyzed from budded cells, as described in Materials and methods. SRL1 mRNA localization was analyzed and quantified from ssd1Δ cells (FLY3196) containing Ssd1 plasmids or empty vector. Cells expressing wild-type Ssd1 (SSD1; FLE1083), Ssd1-9D (SSD1-9D; FLE1087), and empty vector (ssd1Δ; pRS415) exhibited five general patterns of SRL1 mRNA localization, as depicted in the graph and representative images. These include cells with no SRL1 mRNA spots (no spots), multiple faint spots, and 1–3 bright spots restricted to the mother (M), bud (D), or both mother and bud (M + D). Data from three independent experiments were quantified. Each experiment yielded the same results and indicated that SRL1 mRNA was significantly less polarized in ssd1Δ cells (fewer cells with SRL1 mRNA restricted to the bud “D”) than in corresponding SSD1 and SSD1-9D cells. 95% confidence intervals are represented by the solid black lines. Non-overlapping confidence intervals indicate statistically significant differences at significance level 0.05. Each image represents a single optical section. Bar, 2 µm.