Figure 5. In vivo expression of Ssd1-9A and Ssd1-9D. (A) Transformants of ssd1Δ (FLY2184) cells carrying low copy plasmids encoding wild-type Ssd1, Ssd1-9A, or Ssd1-9D (FLE1079, FLE1080, FLE1081) under the control of the physiological SSD1 promoter. (B) 10-fold dilution series of three separate transformants (primary colonies) for each plasmid were spotted onto plates and incubated at 22 or 37°C. Note the poor growth for the Ssd1-9A cells at 22°C and the lethality at 37°C.
Figure 5.

In vivo expression of Ssd1-9A and Ssd1-9D. (A) Transformants of ssd1Δ (FLY2184) cells carrying low copy plasmids encoding wild-type Ssd1, Ssd1-9A, or Ssd1-9D (FLE1079, FLE1080, FLE1081) under the control of the physiological SSD1 promoter. (B) 10-fold dilution series of three separate transformants (primary colonies) for each plasmid were spotted onto plates and incubated at 22 or 37°C. Note the poor growth for the Ssd1-9A cells at 22°C and the lethality at 37°C.

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