Telomere hypercluster formation and maintenance depends on Sir3 levels. (A) Expression levels of Sir3 upon induction. Immunoblots with anti-Sir3 and anti-Pgk1 (loading control) on crude extracts from wild type (WT; yAT7), GALSp-SIR3 (yAT369), and GAL1p-SIR3 (yAT370). Graphical representation of the quantification of Sir3 levels during the time course in the GALSp-SIR3 and GAL1p-SIR3 strains. Sir3 amounts are normalized to endogenous levels. (B) Fluorescent images of wild-type (yAT7), GALSp-SIR3 (yAT369), and GAL1p-SIR3 (yAT370) cells after 8 h in galactose medium. Numbers correspond to the mean intensity of foci. Bar, 2 µm. (C) Quantification of images from B using Q-foci. (D) Graphical representation of the foci intensity as a function of the number of foci per cells. Horizontal bars represent the medians for each category of cells.