Actin body mobilization requires glucose catabolism. (A) Schematic representation of the glycolysis pathway. Genes encoding each activity in S. cerevisiae are indicated in green. G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; F1,6BP, fructose 1,6 bisphosphate; DHAP, dihydroxy acetone phosphate; G3P, glyceraldehyde 3 phosphate; 1,3BPG, 1,3 bis phosphoglycerate; 3-PG, 3 phosphoglycerate; 2-PG, 2 phosphoglycerate; PEP, phosphoenolpyruvate. (B) WT, snf3Δ, rgt2Δ, gpr1Δ (Δsensors) and hxk1Δ, hxk2Δ, and glk1Δ (Δhexokinases) strains were grown in galactose-rich medium. WT and a strain that cannot uptake glucose (Δtransporters strain; Wieczorke et al., 1999) were grown in maltose-rich medium. WT and pgi1Δ mutant were grown in fructose-rich medium. WT, pfk1Δ, pfk2Δ double mutant, and pgk1Δ mutants were grown in lactate-rich medium. After 7 d, cells were transferred into glucose (2%) and the actin cytoskeleton remodeling was monitored by phalloidin staining. (C) UV chromatograms from WT cells grown for 7 d in YPDA (black line), then transferred for 15 min into 2% glucose (red line) or into water (green line). Blue insets show enlarged views of the of AICAR and SAICAR peaks, the black inset shows amperometric detection of G6P and F1,6BP. (D and E) WT and pgi1 strains were grown for 7 d in rich medium containing 2% fructose, then transferred into either a 2% glucose or fructose solution. (D) ATP, G6P, and F1,6BP concentration measured before (7 d) and 15 min after transfer. (E) Actin cytoskeleton organization revealed by phalloidin staining. For each time point, n > 200 cells, at least two experiments. Error bars indicate SEM.