Figure 3. Quiescence exit can be triggered independently of reentry into the proliferation cycle. (A) WT cells were grown for 7 d in YPDA then transferred into the indicated medium. The budding index and the actin cytoskeleton organization are shown. (B) WT cells expressing Pre4p, a proteasome subunit, fused to GFP, were grown for 7 d in YPDA then transferred into the indicated medium. For each time point, the budding index and proteasome localization were monitored. (C) S. pombe expressing Pad1-GFP, a proteasome subunit, fused to GFP, were grown for 4 d and then transferred into the indicated medium. For each time point, proteasome localization was monitored. Cell outlines are drawn in white. n > 200 cells, two experiments. Errors bars indicate SEM. Bars, 2 µm.
Figure 3.

Quiescence exit can be triggered independently of reentry into the proliferation cycle. (A) WT cells were grown for 7 d in YPDA then transferred into the indicated medium. The budding index and the actin cytoskeleton organization are shown. (B) WT cells expressing Pre4p, a proteasome subunit, fused to GFP, were grown for 7 d in YPDA then transferred into the indicated medium. For each time point, the budding index and proteasome localization were monitored. (C) S. pombe expressing Pad1-GFP, a proteasome subunit, fused to GFP, were grown for 4 d and then transferred into the indicated medium. For each time point, proteasome localization was monitored. Cell outlines are drawn in white. n > 200 cells, two experiments. Errors bars indicate SEM. Bars, 2 µm.

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