Cells can enter quiescence in G2/M upon glucose exhaustion. (A) Diagram representing the experimental protocol: WT and cdc13-1 rho⁰ cells were grown in YPDA at 25°C. At OD_600nm 2, cells were transferred to 37°C for 14 h, then transferred back to 25°C. Budding indexes for WT and cdc13-1 rho⁰ strains are shown. (B) Actin cytoskeleton organization in WT and cdc13-1 rho⁰ before and after glucose exhaustion, respectively, 6 and 32 h after the shift to 37°C. Bars, 2 µm. (C) Whi5p-GFP localization in WT (green) and cdc13-1 rho⁰ cells (blue). WT and cdc13-1 rho⁰ Whi5p-GFP–expressing cells were grown in YPDA at 25°C. At OD_600nm 2, cells were transferred to 37°C or to 25°C for 14 h. The budding index is indicated for each time point (WT, green triangles; cdc13-1 rho⁰, blue circles). Glucose exhaustion is indicated. n > 200 cells for each time point, two experiments. Errors bars indicate SEM.