Figure 2. fob mutants exhibit a defect in bacterial clearance. (A) 2 h after injection, pHrodo-labeled E. coli (arrows) were visible around the dorsal vessel in the thorax of wild-type and eater flies (Df(3R)D605/Df(3R)Tl-I). In contrast, diffuse weaker signals appeared in fob1 flies. (B) Hemocytes were allowed to engulf FITC-labeled E. coli for 15 min, and, after quenching fluorescence of external bacteria with Trypan blue, the fluorescence of phagocytosed bacteria was visible in wild-type (B) and fob1 (C) cells visualized by differential interference contrast microscopy (B′ and C′). (D and E) After 45 min of chase, bacteria were cleared from wild type (D) but accumulated inside fob1 hemocytes (E). (F) Box and whisker plots display the number of bacteria detected in hemocytes of indicated genotypes after 45 min of chase. Bars: (A) 0.5 mm; (B and C) 25 µm; (D and E) 10 µm.
Figure 2.

fob mutants exhibit a defect in bacterial clearance. (A) 2 h after injection, pHrodo-labeled E. coli (arrows) were visible around the dorsal vessel in the thorax of wild-type and eater flies (Df(3R)D605/Df(3R)Tl-I). In contrast, diffuse weaker signals appeared in fob1 flies. (B) Hemocytes were allowed to engulf FITC-labeled E. coli for 15 min, and, after quenching fluorescence of external bacteria with Trypan blue, the fluorescence of phagocytosed bacteria was visible in wild-type (B) and fob1 (C) cells visualized by differential interference contrast microscopy (B′ and C′). (D and E) After 45 min of chase, bacteria were cleared from wild type (D) but accumulated inside fob1 hemocytes (E). (F) Box and whisker plots display the number of bacteria detected in hemocytes of indicated genotypes after 45 min of chase. Bars: (A) 0.5 mm; (B and C) 25 µm; (D and E) 10 µm.

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