Nuclear shape change in epidermal cells of sun1-KO sun2-KD and wip1-1 wip2-1 wip3-1 plants. (A) Comparison of nuclear shapes in trichomes, leaf epidermal cells, and mature root hair cells of wild type, sun1-KO sun2-KD, and wip1-1 wip2-1 wip3-1. Nuclei were DAPI stained, and mature root hair nuclei images in the bottom row are confocal maximum intensity projections using GFP-NLS-GFP as a nuclear marker. (B) Quantitative comparison of nuclear shape changes shown in A. Asterisks indicate significant statistical difference (P < 0.01, using a t test; n = 60 for leaf epidermal cells; n = 20 for trichomes; n = 55 for root hairs) compared with wild type. Error bars represent SEM. (C) A confocal maximum intensity projection showing a super-elongated nucleus in a wild-type mature root hair using WPP-GFP as an NE marker. Bars, 10 µm.