Figure 3.
Figure 3. FRAP analysis of the interaction between AtWIP1 and AtSUN1. (A) Recovery curves of GFP-AtWIP1, GFP-AtWIP1ΔVVPT, and GFP-TDFAtWIP1. (B) Recovery curves of GFP-AtWIP1 coexpressed with RFP-Flag-AtSUN1 or RFP-Flag-AtSUN1ΔNSUN. (C) Recovery curves of GFP-AtWIP1 coexpressed with RFP-Myc-AtSUN2 or RFP-Myc-AtSUN2ΔNSUN. (D) Recovery curves of GFP-AtWIP1ΔVVPT coexpressed with RFP-Flag-AtSUN1 or RFP-Myc-AtSUN2. (E) Recovery curves of GFP-TDFAtWIP1 coexpressed with RFP-Flag-AtSUN1 or RFP-Myc-AtSUN2. (A–E) Error bars represent SEM (n = 60 for GFP-AtWIP1 in C; n = 30 for all others). Asterisks at the end of each curve indicate significant statistical difference of the maximum recovery compared with the green curve in each figure (P < 0.01, using a t test). Otherwise, no statistical difference has been observed (P > 0.05, using a t test).

FRAP analysis of the interaction between AtWIP1 and AtSUN1. (A) Recovery curves of GFP-AtWIP1, GFP-AtWIP1ΔVVPT, and GFP-TDFAtWIP1. (B) Recovery curves of GFP-AtWIP1 coexpressed with RFP-Flag-AtSUN1 or RFP-Flag-AtSUN1ΔNSUN. (C) Recovery curves of GFP-AtWIP1 coexpressed with RFP-Myc-AtSUN2 or RFP-Myc-AtSUN2ΔNSUN. (D) Recovery curves of GFP-AtWIP1ΔVVPT coexpressed with RFP-Flag-AtSUN1 or RFP-Myc-AtSUN2. (E) Recovery curves of GFP-TDFAtWIP1 coexpressed with RFP-Flag-AtSUN1 or RFP-Myc-AtSUN2. (A–E) Error bars represent SEM (n = 60 for GFP-AtWIP1 in C; n = 30 for all others). Asterisks at the end of each curve indicate significant statistical difference of the maximum recovery compared with the green curve in each figure (P < 0.01, using a t test). Otherwise, no statistical difference has been observed (P > 0.05, using a t test).

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