Inactivation of cby leads to defective behavior and mechanosensation. (A) Diagram of the cby genomic locus. The second coding exon was replaced by homologous recombination with a miniwhite gene flanked by two LoxP sites. The primers designed to screen for the recombinant events are indicated in yellow for the left arm and red for the right arm. (B) Diagram representing the bang assay to evaluate fly coordination. The number of flies that reach a defined level in the tube is counted 1 min after tapping the tube. cby1/1 flies and cby1/Df(3R)BSC805 (cby1/Df) stay at the bottom of the tube compared with control flies. The phenotype is rescued by adding one copy of the cby-Tomato transgene. n = 30 flies per assay. Error bars represent the SD of five assays. (C) Antennal nerve potentials recorded from age-matched sib cby/TM6C heterozygotes (cby/+), cby homozygotes (cby/cby), and from Df(3R)BSC805/TM6B (Df/TM6B) and cby/Df(3R)BSC805 (cby/Df) sibs in response to a five-pulse sound stimulus (stim). Each trace represents the averaged responses to 10 stimuli. Traces shown are those closest to the mean values for their respective genotypes. The third trace is representative of mutant recordings that do not show discernible SEP peaks. (D) Summary of sound-evoked nerve potential amplitudes. Mean and median peak amplitudes of the antennal SEPs for each indicated genotype. Mean values are derived only from those recordings showing discernible peaks; medians are from all recordings.
