The Moody/GPCR pathway regulates myosin association with the ARSs. (A–F) Nerve cords dissected from moody^Δ17 mutant larvae and also carrying either Sqh-GFP (A–C) or Nrg-GFP (D–F). GFP labeling is shown in A and D, and phalloidin labeling is shown in B and E. C and F are their corresponding merged images. The arrow in A shows a complete dissociation of Sqh-GFP from the ARS and in B abnormal morphology of the ARSs. The arrowhead in D shows discontinuity of Nrg-GFP labeling, and the arrows in D and E show corresponding locations of abnormal ARS morphology. (G–I) Cross section of third instar larvae nerve cord expressing moesin-GFP and labeled with Moody-α (red). A partial overlap between the moesin-GFP and Moody-α is detected. Moody staining wraps the ARS (arrows).