Figure 7. Effect of altered phosphoinositide metabolism on the binding of IgG-opsonized targets. (A) RAW macrophages were transfected with plasmids expressing a form of human type IV phosphoinositide-5 phosphatase (5-Pase) that is RFP tagged and can be targeted to the membrane upon addition of rapamycin (Rap). Shown is the effect of addition of 1 µM Rap or vehicle (DMSO) only on the recruitment of the 5-Pase to the membrane. (B) Binding of IgG-opsonized beads to cells transfected with the recruitable 5-Pase after treatment with DMSO or Rap. (C and D) Effect of 100 µM LY294002 on the distribution of Akt-PH-RFP (C) and on IgG-coated bead binding (D). The images are representative of four independent experiments; bar, 17 µm. Data in B and D are normalized means ± SEM of three independent experiments, each counting the beads bound by 150 macrophages.
Figure 7.

Effect of altered phosphoinositide metabolism on the binding of IgG-opsonized targets. (A) RAW macrophages were transfected with plasmids expressing a form of human type IV phosphoinositide-5 phosphatase (5-Pase) that is RFP tagged and can be targeted to the membrane upon addition of rapamycin (Rap). Shown is the effect of addition of 1 µM Rap or vehicle (DMSO) only on the recruitment of the 5-Pase to the membrane. (B) Binding of IgG-opsonized beads to cells transfected with the recruitable 5-Pase after treatment with DMSO or Rap. (C and D) Effect of 100 µM LY294002 on the distribution of Akt-PH-RFP (C) and on IgG-coated bead binding (D). The images are representative of four independent experiments; bar, 17 µm. Data in B and D are normalized means ± SEM of three independent experiments, each counting the beads bound by 150 macrophages.

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