Figure 2. Stimulation of γ-TuRC for microtubule nucleation. (A) After immunoprecipitation of ectopically expressed proteins through the tag moiety (i.e., Flag), the immunoprecipitates and cell lysates were examined for γ-tubulin and GCP5. WT, 51–100 wild type; F75A, 51–100 (F75A). (B and C) Microtubule polymerization was performed with or without the isolated γ-TuRC and 51–200 (B) or the entire CDK5RAP2 protein (C). Representative microscopic fields of polymerized microtubules are shown. Microtubules were counted from 20 random fields to derive the mean numbers of microtubules. Data are shown as mean ± SD of three independent experiments. Bar, 10 µm.
Figure 2.

Stimulation of γ-TuRC for microtubule nucleation. (A) After immunoprecipitation of ectopically expressed proteins through the tag moiety (i.e., Flag), the immunoprecipitates and cell lysates were examined for γ-tubulin and GCP5. WT, 51–100 wild type; F75A, 51–100 (F75A). (B and C) Microtubule polymerization was performed with or without the isolated γ-TuRC and 51–200 (B) or the entire CDK5RAP2 protein (C). Representative microscopic fields of polymerized microtubules are shown. Microtubules were counted from 20 random fields to derive the mean numbers of microtubules. Data are shown as mean ± SD of three independent experiments. Bar, 10 µm.

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