Cyclin A2 is differentially expressed in primary human tumors versus metastases. (A and B) Cyclin A2 quantification in SW480 and SW620 cells (A; *, P = 0.031) or matched human primary colorectal tumors (T) and their corresponding metastases (M) from 12 individuals (B; ****, P > 0.0001). Identical amounts of protein extracts were loaded. Data are means ± SEM. (C) Three representative examples of immunohistological Cyclin A2 staining in primary colorectal tumors (middle) and their metastases (bottom) versus normal tissue (top). (inset) Enlargement of the indicated region shows cytoplasmic Cyclin A2 staining. Bars, 100 µm. (D) Quantification was performed on eight matched samples (**, P = 0.0055). Molecular markers are given in kilodaltons. Bars (whiskers) represent maximum and minimum values; the white line corresponds to the median.