Rearrangement of Actin cytoskeleton upon Cyclin A2 depletion is independent of Cdk1 and Cdk2 activities. (A) F-Actin (red) and Cyclin A2 (green) staining by phalloidin or anti-Flag antibody of Cdk2^−/− cells treated with the Cdk1 inhibitor RO 3306. Cdk2^−/− cells were infected with CycA2 shRNA and transfected with wild-type (WT) Cyclin A2. Bars, 20 µm. (B) FACS analysis of cell cycle distribution after treatment of Cdk2^−/− cells with RO 3306 for 24 h. Data are represented as means ± SEM (n = 3). (C) Western blot analysis of Cdk2 and Cyclin A2 after infection with CycA2 shRNA in Cdk2^−/− cells. GAPDH is used as a loading control. Molecular markers are given in kilodaltons.