Actin colocalizes with a subset of junctional complexes in polarized MDCK cells. (a–d) Electron micrographs showing adherens junction (white arrow) and numerous interactions between adjacent cells along the lateral membrane (arrowheads; a). Cell–cell interactions are at apical (b), lateral (c), and basal membranes (d) of cells. Bars, 500 nm. (e and f) Single deconvolved optical z slices at the apical region of cells. (g and h) Projection of 35 deconvolved optical z slices spanning the apical 7 µm of cells. (i and j) Projection of the next 10 deconvolved optical z slices spanning 2 µm of the basal region of cells. (e–j) Yellow arrowheads point to areas where α-catenin (blue), β-catenin (red), and actin (phalloidin; green) are colocalized. White arrowheads point to areas where actin is missing from the α- and β-catenin puncta. Bars, 2 µm.