Figure 3. F-actin localization at the division site in N. crassa. (A) The actin-binding probe Lifeact-GFP accumulated along the hyphal cortex, assembled into a contractile ring, and persisted at the ring throughout septation. The time is indicated in minutes. Bar, 10 µm. See also Video 2. (B) Actomyosin ring constriction was observed using Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane, and cell wall, respectively. Image shows a transverse section of a representative cell during ring constriction. Bar, 5 µm. See also Video 3. (C) A kymograph showing the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red) and a merge of the two.
Figure 3.

F-actin localization at the division site in N. crassa. (A) The actin-binding probe Lifeact-GFP accumulated along the hyphal cortex, assembled into a contractile ring, and persisted at the ring throughout septation. The time is indicated in minutes. Bar, 10 µm. See also Video 2. (B) Actomyosin ring constriction was observed using Lifeact-GFP (green), FM4-64 (red), and calcofluor (blue) to label actin, cell membrane, and cell wall, respectively. Image shows a transverse section of a representative cell during ring constriction. Bar, 5 µm. See also Video 3. (C) A kymograph showing the decrease in ring diameter over time within a representative hypha expressing Lifeact-GFP (green) and membranes labeled with FM4-64 (red) and a merge of the two.

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