Advanced state of formation of densely spaced N-cadherin–containing AJs in a culture of human hepatocellular carcinoma cells. Double-label laser-scanning confocal immunofluorescence microscopy of a reformed PLC monolayer as visualized after reaction with antibodies against N-cadherin (red) located in the numerous, very densely spaced puncta adharentia, which in many regions suggest a continuous AJ structure in a fully developed cytoskeletal system (keratin fibril bundles are shown after reaction with antibodies to keratins 8 and 18; green). Bar, 20 µm.