RSC2 deletion does not affect Cdc5 chromosomal distribution. Wild-type (wt; ySP7797) and rsc2Δ cells (ySP8200) expressing FLAG-tagged Cdc5 (A) and wild-type cells expressing PK-tagged Scc1 (B) were arrested in mitosis with benomyl and treated for ChIP-on-chip analysis. Enrichment of DNA fragments in the immunoprecipitate relative to a whole-genome DNA sample is shown along the first 160 kb (left arm and centromere) of chromosome VI. The y-axis scale is log2. Orange signals represent significant binding as previously described (Katou et al., 2003). The used statistical algorithm is identical to that for the GeneChip Operating Software (Affymetrix). The greenish signal indicates the centromere. Blue bars above and below the midline represent ORFs transcribed from left to right and opposite, respectively. A region around 140 kb masked by a gray box corresponds to Ty retrotransposon, which exists in multiple copies in the genome and was omitted from the analysis.