Cell cycle progression of rsc2Δ cells and their recovery from SAC activation. (A) Cultures of wild-type (wt; ySP4806) and rsc2Δ (ySP6997) cells were grown in YEPD, arrested in G1 by α-factor, and then released in fresh medium (t = 0). At the indicated times, samples were analyzed as in Fig. 1 A. (B) The same strains as in A were grown in YEPD, arrested in mitosis by 5 µg/ml nocodazole treatment, and then released (t = 0) in 10 µg/ml YEPD containing α-factor, followed by the same analyses as in Fig. 1 A.