![Figure 4. Boi acts through the canonical Hh pathway. (A and B) Reducing Hh expression in all cells (hhAC/+) or in apical cells (hhRNAi/bab-Gal4) suppresses boi mutant defects. (A) The mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (wild-type [WT] or bab-Gal4/+, P < 0.00000006). **, significant differences relative to boie or boie; babGal4/+ (P < 0.000001). (B) Mean numbers of dividing FSCs (PH3+) per germarium are shown for genotypes indicated. *, significant differences relative to control (WT or bab-Gal4/+, P < 0.0002). **, significant differences relative to boie or boie; bab-Gal4/+ (P < 0.02). (C and D) Reducing Smo or Ci expression in all cells (smo3/+) or in FSCs and their progeny (smoRNAi/109-30-Gal4, or CiRNAi/109-30-Gal4) suppresses boi mutant defects. (C) Mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (WT or 109-30/+, P < 0.0000009). **, significant differences relative to boie or boie;109-30/+ (P < 0.00001). (D) Mean numbers of dividing FSCs per germarium (PH3+) are shown. *, significant differences relative to control (WT or 109-30/+, P < 0.007). **, significant differences relative to boie or boie;109-30/+ (P < 0.04). (E) Wild-type germaria immunostained with antibodies against Smo (green), Vasa (germ cells, blue), and Hh (red). Brackets indicate apical cells. (F) Mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (babGal4/+ or 109-30/+, P < 0.004). **, significant differences relative to boie; babGal4/+, P < 0.0000002). (G) Expression of BoiΔFN2 (green) in boi mutant apical cells (boie; UAS-boiΔFN2/+; babGal4/+) rescues Hh localization (red). Error bars represent SEM.](https://cdn.rupress.org/rup/content_public/journal/jcb/191/5/10.1083_jcb.201007142/4/jcb_201007142_rgb_fig4.jpeg?Expires=1767691995&Signature=j34JbRDq2G8lBTuqhbTOWy5QG2zAOeSIbDzXLaKvz7-mJ97w~RwPU3KhREMBQKqudOXaC3kjcT5ZotuchTk6BIXDkB0CuxWCOPYbWG3m6es8xejQpd~z~2qCYTKDEZXiqW6lGlxHDtc6-5Km0yuVihFXw~jQMlyckcHFf1bFU-wI-L-dPjR-IHOUaJ0oFkWbObPJuZRcPxrUweF5iAyQdYQ6fRtskq~Ylfy76fPIU0NKOr~CWIz1fh3KIbVXKf4q~T3pb83dSBd4I43NB0SjbEGTdZ-t6lAnD1wrtpJv~3yz~QT5NzG19maDwta6MJ4nMfOnQVgv9Z3YNPBnuZrgIQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Boi acts through the canonical Hh pathway. (A and B) Reducing Hh expression in all cells (hhAC/+) or in apical cells (hhRNAi/bab-Gal4) suppresses boi mutant defects. (A) The mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (wild-type [WT] or bab-Gal4/+, P < 0.00000006). **, significant differences relative to boie or boie; babGal4/+ (P < 0.000001). (B) Mean numbers of dividing FSCs (PH3+) per germarium are shown for genotypes indicated. *, significant differences relative to control (WT or bab-Gal4/+, P < 0.0002). **, significant differences relative to boie or boie; bab-Gal4/+ (P < 0.02). (C and D) Reducing Smo or Ci expression in all cells (smo3/+) or in FSCs and their progeny (smoRNAi/109-30-Gal4, or CiRNAi/109-30-Gal4) suppresses boi mutant defects. (C) Mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (WT or 109-30/+, P < 0.0000009). **, significant differences relative to boie or boie;109-30/+ (P < 0.00001). (D) Mean numbers of dividing FSCs per germarium (PH3+) are shown. *, significant differences relative to control (WT or 109-30/+, P < 0.007). **, significant differences relative to boie or boie;109-30/+ (P < 0.04). (E) Wild-type germaria immunostained with antibodies against Smo (green), Vasa (germ cells, blue), and Hh (red). Brackets indicate apical cells. (F) Mean cell number in the first stalk for each genotype is shown. *, significant differences relative to control (babGal4/+ or 109-30/+, P < 0.004). **, significant differences relative to boie; babGal4/+, P < 0.0000002). (G) Expression of BoiΔFN2 (green) in boi mutant apical cells (boie; UAS-boiΔFN2/+; babGal4/+) rescues Hh localization (red). Error bars represent SEM.
