Bni1-dependent suppression of endocytosis and growth defects in adaptor mutant cells grown with osmotic support. (A) WT, 4Δ+Ent1, and 4Δ+ENTH1 cells expressing Ste3-GFP were grown on synthetic medium in the absence (−sorbitol) or presence (+ 1 M sorbitol) of osmotic support as indicated and imaged by fluorescence microscopy. bni1Δ and bnr1Δ (B and C, respectively) in WT, 4Δ+Ent1, and 4Δ+ENTH1 adaptor backgrounds were assessed for Ste3-GFP localization in the absence or presence of osmotic support as in A. The inset shows additional cells at the same magnification. Bars, 2.5 µm. (D) Growth of strains used in A–C at permissive (30°C) and nonpermissive (37°C) temperatures in the absence (YPD) or presence (YPD + 1 M sorbitol) of osmotic support.