Rho1-mediated endocytosis does not restore cortical actin patch dynamics, and functions in the absence of clathrin. (A) Kymograph analysis of WT, 4Δ+Ent1, and 4Δ+ENTH1 cells expressing GFP-tagged Ede1, Pan1, or Las17 with empty vector or Rom1 as indicated. Images were collected every 4 s (480 s total) for Ede1, every 3 s (360 s total) for Pan1, and every 2 s (240 s total) for Las17. (B) Ste3-pHluorin localization was assessed in chc1Δ cells with empty vector, Yap1801, or Rom1 as indicated. Insets show additional cells at the same magnification. Bar, 2.5 µm. (C) Quantification of Ste3-pHluorin intensity (a.u.; error bars indicate mean ± SEM; ***, P < 0.001) in cells from B.