Accumulation of actin module components in aberrant comet tail structures. (A) 4Δ+Ent1 and 4Δ+ENTH1 cells expressing GFP-tagged Las17, Sac6, Rvs167, or Sjl2 and Abp1-mCherry were assessed for colocalization of GFP (green) and mCherry (red) signals. Projection images from 0.25-µm-step z stacks are shown. (B) Projection images of WT, 4Δ+Ent1, and 4Δ+ENTH1 strains expressing Sla1-GFP and Abp1-mCherry with empty vector or Rom1 as indicated. Insets show additional cells at the same magnification. Bars, 2.5 µm.