Requirement of actin cable stabilization, but not actin polymerization or stabilization at cortical patches, for Rho1-mediated endocytosis. (A and B) Ste3-GFP localization was assessed in las17Δ (A) and sac6Δ scp1Δ (B) cells with empty vector, Yap1801, or Rom1 plasmids. (C) tpm1Δ was generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 cells, and Ste3-GFP localization was assessed in strains with empty vector or Rom1 as indicated. Quantification of Ste3-pHluorin intensity (a.u.; error bars indicate mean ± SEM; *, P < 0.05; ***, P < 0.001) is shown to the right of each group of Ste3-GFP images. Insets show additional cells at the same magnification. Bars, 2.5 µm.