Requirement of polarisome components for Rho1-mediated endocytosis. spa2Δ and bud6Δ strains were generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 backgrounds with empty vector or Rom1 plasmids as indicated. Ste3-GFP localization was assessed by fluorescence microscopy. Quantification of Ste3-pHluorin intensity (a.u.; error bars indicate mean ± SEM) is shown to the right of each group of Ste3-GFP images. The inset shows additional cells at the same magnification. Bar, 2.5 µm.