Figure 5.
![Figure 5. Contribution of Bni1 protein–protein interaction regions to Rho1-mediated endocytosis. (A) Schematic of full-length and truncated Bni1 fragments used. Bni1 fragments were expressed from the BNI1 promoter. (B) Ste3-GFP was examined by fluorescence microscopy in bni1Δ 4Δ+Ent1 and bni1Δ 4Δ+ENTH1 cells with empty vector, full-length or truncated Bni1 fragments [CEN TRP1], and empty vector or high-copy Rom1 [2μ URA3] plasmids as indicated. Bar, 2.5 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/195/4/10.1083_jcb.201104045/4/jcb_201104045_gs_fig5.jpeg?Expires=1767775742&Signature=UMeUzPYIh1Q4NiR5QhY9BAWVrNHixf1YWY9aq2ESfSsIzqBCFxGxzTgF0n943A4hJ8vQKULK9uzrdg8~Z-lvxXPDcU3i3HEr5NYoF1BcjYiS9aW0k4yiP0R1Ms-j8yVLlqgaTBrIP5xZeeg4YCjCQu8IdjySbYrQAhzguPUCmV17MygImy06J6iqtq7g7CWoMV5W6mFzEe7kyZjtdRR2C1QP6dFLI5Rr4UVOLYz1uh91EOPGwZVrcV2KRwHJvOXiUgnT67KkuWKhliGs9RdKinG-M0BEQc8tTpGsFkKobLYmaKBVX2gGG2bfc51-2yHbnc2yP8S3z-UhjCRQ8YgcLw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Contribution of Bni1 protein–protein interaction regions to Rho1-mediated endocytosis. (A) Schematic of full-length and truncated Bni1 fragments used. Bni1 fragments were expressed from the BNI1 promoter. (B) Ste3-GFP was examined by fluorescence microscopy in bni1Δ 4Δ+Ent1 and bni1Δ 4Δ+ENTH1 cells with empty vector, full-length or truncated Bni1 fragments [CEN TRP1], and empty vector or high-copy Rom1 [2μ URA3] plasmids as indicated. Bar, 2.5 µm.
