Figure 4.
Figure 4. Effect of Rho1 effector deletion on endocytosis mediated by the Rho1 pathway. (A) Ste3-GFP was visualized by fluorescence microscopy in sec3Δ, fks1Δ, skn7Δ, and bni1Δ strains generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 backgrounds with empty vector or Rom1 plasmids as indicated. (B) Ste3-GFP localization was assessed by fluorescence microscopy in bnr1Δ strains generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 backgrounds with empty vector or Rom1 plasmids. Quantification of Ste3-pHluorin intensity (a.u.; error bars indicate mean ± SEM; ***, P < 0.001) is shown to the right of each group of images. Bars, 2.5 µm.

Effect of Rho1 effector deletion on endocytosis mediated by the Rho1 pathway. (A) Ste3-GFP was visualized by fluorescence microscopy in sec3Δ, fks1Δ, skn7Δ, and bni1Δ strains generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 backgrounds with empty vector or Rom1 plasmids as indicated. (B) Ste3-GFP localization was assessed by fluorescence microscopy in bnr1Δ strains generated in WT, 4Δ+Ent1, and 4Δ+ENTH1 backgrounds with empty vector or Rom1 plasmids. Quantification of Ste3-pHluorin intensity (a.u.; error bars indicate mean ± SEM; ***, P < 0.001) is shown to the right of each group of images. Bars, 2.5 µm.

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