Figure 3.
Figure 3. The Rho1 pathway mediates actin-dependent internalization from the plasma membrane. WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector or Rom1 were treated with 200 µM LatA or an equivalent volume of DMSO for 2 h before visualization of Ste3-GFP by fluorescence microscopy. The inset shows additional cells at the same magnification. Bar, 2.5 µm.

The Rho1 pathway mediates actin-dependent internalization from the plasma membrane. WT, 4Δ+Ent1, and 4Δ+ENTH1 cells with empty vector or Rom1 were treated with 200 µM LatA or an equivalent volume of DMSO for 2 h before visualization of Ste3-GFP by fluorescence microscopy. The inset shows additional cells at the same magnification. Bar, 2.5 µm.

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